ການຜະລິດແບັກທີເຣຍນາໂນເຊລລຸຍໂລສຈາກ Komagataeibacter intermedius ທີ່ອຸນຫະພູມສູງ

Abstract

ເຊລລຸຍໂລສເປັນໂພລີເມີສາຍຊື່ທີ່ປະກອບດ້ວຍກຼູໂຄສມາຕໍ່ກັນດ້ວຍສໍາພັນໄກລໂກໄຊດ໌ (glycosidic bond) ທີ່ຕໍາແໜ່ງເບຕ້າ-1,4 ເຊິ່ງເປັນສ່ວນປະກອບທີ່ສໍາຄັນຂອງຂອບຮູບຈຸລັງຂອງພືດ, ສ່ວນເຊລລຸຍໂລສທີ່ໄດ້ຈາກພືດມັກມີໂພລີເມີຊັກຄາໄລດ໌ຊະນິດອື່ນໆ ປົນເປື້ອນມານໍາ ເຊັ່ນ: ລິກນິນ ແລະ ເຮມີເຊລລຸຍໂລສ. ດັ່ງນັ້ນ, ເຊລລຸຍໂລສຈາກແບັກທີເຣຍຈິ່ງເປັນທາງເລືອກໜື່ງໃນບັນດາແຫຼ່ງເຊລລຸຍໂລສທີ່ມີຄວາມບໍລິສຸດສູງ. ຜົນການທົດສອບການຜະລິດເຊລລຸຍໂລສຈາກນໍ້າໝາກໄມ້ 3 ຊະນິດ ຄື: ນໍ້າໝາກພ້າວ, ນໍ້າ ໝາກນັດ ແລະ ໝາກຂາມປ້ອມ ທີ່ຊື້ມາຈາກ 3 ແຂວງ ຄື: ແຂວງຫຼວງພະບາງ, ແຂວງວຽງຈັນ ແລະ ແຂວງຄໍາມ່ວນ ໂດຍໃຊ້ເຊື້ອ SNL252 Komagataeibacter intermedius ທີ່ແຍກໄດ້ຈາກໝາກກ້ວຍ ເພື່ອນໍາມາທົດສອບການໝັກ ໂດຍປຽບທຽບສູດອາຫານທີ່ຕື່ມ 1% acetic acid and 2% ethanol (CW1A2E), (PA1A2E), (GB1-3-5-1A2E)  ກັບສູດອາຫານທີ່ເພີ່ມ 0.5% sucrose  and 0.5% ammonium sulfate (CW1A2E0.5S0.5N), (PA1A2E0.5S0.5N), (GB1-3-5-1A2E0.5N) ແລະ ນໍາໄປບົ່ມທີ່ອຸນຫະພູມຫ້ອງ ແລະ 37 ºC ເປັນເວລາ 2 ອາທິດ ແລ້ວນໍາມາຫາປະລິມານນໍ້າໜັກແຫ້ງຂອງເຊລລຸຍໂລສທີ່ຜະລິດໄດ້ໃນອາຫານໝັກແຕ່ລະປະເພດ. ການທົດລອງຄັ້ງນີ້ແມ່ນເຮັດສາມຊໍ້າ ແລະ ຂໍ້ມູນທີ່ໄດ້ແມ່ນໃຊ້ໂປຼແກມ Microsoft Excel  ໃນການວິເຄາະ. ຈາກຜົນການທົດລອງພົບວ່າ ສູດອາຫານທີ່ເໝາະສົມຂອງເຊື້ອ K. intermedius ເພື່ອການໝັກເຊລລຸຍໂລສໄດ້ແກ່ ສູດອາຫານທີ່ເຕີມ sucrose ແລະ ແອັມໂມເນຍຊັນເຟດ. ສ່ວນອຸນຫະພູມທີ່ເໝາະສົມ ໃນການໝັກເຊລລຸຍໂລສ ແມ່ນ  37 ºC. ຜົນຈາກການປຽບທຽບສູດອາຫານໝັກຈາກໝາກໄມ້ 3 ຊະນິດ ທີ່ເຕີມ sucrose ແລະ ແອັມໂມເນຍຊັນເຟດ ທີ່ອຸນຫະພູມ 37 ºC ພົບວ່າ ສູດອາຫານນໍ້າໝາກພ້າວຈາກແຂວງຄໍາມ່ວນສາມາດຜະລິດໄດ້ສູງກວ່າໝູ່ເຖິງ 5.49 g/L ຮອງລົງມາ ແມ່ນ ນໍ້າໝັກໝາກຂາມປ້ອມຈາກແຂວງວຽງຈັນສາມາດຜະລິດເຊລລຸຍໂລສໄດ້ 4.12 g/L ແລະ ໄດ້ຜົນຜະລິດໜ້ອຍກວ່າໝູ່ແມ່ນສູດອາຫານນໍ້າໝາກນັດ ສາມາດຜະລິດໄດ້ພຽງ 2.43 g/L.

Cellulose is a linear glucose polymer of ß-1,4-glycosidic bond, which is the main component of plant cells walls. However, cellulose from plant is still contaminated with polysaccharides, such as lignin and hemicellulose. Thus, bacterial cellulose is alternative sources of high purity cellulose that consist only glucose monomer, it exhibits numerous great properties. In this study we aim to  optimization and improve bacterial nanocellulose (BNC) fermentation with low-cost raw materials in large-scale at high temperature by thermotolerant Komagataeibacter intermedius strain isolated from banana was used in this study. The samples were collected from 3 provinces: Luangphrabang, Vientiane and Khammouane for cultivation bacterial BNC fermentation by static cultivation at ambient temperature and 37°C, respectively. The cultivation was carried out in coconut water (CW), pineapple (PA), gooseberry (GB) containing 1% acetic acid and 2% ethanol (CW1A2E), (PA1A2E), (GB1-3-5-1A2E)  compared with CW1A2E, PA1A2E, GB1-3-5-1A2E containing 0.5% sucrose  and 0.5% ammonium sulfate (CW1A2E0.5S0.5N), (PA1A2E0.5S0.5N), (GB1-3-5-1A2E0.5N)  for 14 days.  The yields of the BNC (dry weight) synthesized by Komagataeibacter strains after static cultivation in large-scale at ambient temperature and 37°C for 14 days. These experiments have been done in three replications and using Microsoft excels to analysis. The BNC yield was expressed as grams of BNC dry weight per liter of a cultured medium. The results showed that the suitable temperature for bacterial BNC production was 37°C, respectively.  The results of BNC yield between 3 cultured medium showed that higher BNC producing ability in coconut water from Khammouane province (CW1A2E0.5S0.5N) produced the highest 5.49 g/L, BNC producing ability in gooseberry from Vientiane province (GB1-3-5-1A2E0.5N) produced the highest 4.12 g/L and the BNC producing ability in pineapple from Khammouane province (PA1A2E0.5S0.5N) produced the highest 2.43 g/L under static cultivation at 37°C respectively.