The Surveillance for antibiotic-resistant chickens in Luang Prabang, Lao PDR

Abstract

Salmonella spp. And Escherichia coli has a tendency to be more resistant to antibiotics and resistant to multiple types of antibiotics. The standard method for detecting microorganisms that are resistant to antibiotics were ISO 6579-1:2017 for Salmonella spp and EFSA 2008 for Escherichia coli. The objective of   research to Surveillance the use of antibiotics in chicken cecum the location were Phu Checha Charoen Village and Phu Chang Kham Po Village, Phra Bang Capital, Luang Prabang Province. The samples were cultivated through special medium for 4 days. to obtain pure colony a14 of antimicrobial resistance by using thermos scientific equipment: Sensititre Vizion.

The results found that: The prevalence of Escherichia coli from poultry was 96.53% (139/144), and Salmonella spp. was 36.81% (53/144). The highest rate of Escherichia coli resistance was found in Azithromycin, Ciprofloxacin, and Sulfamethoxazole (100%, 44/44) followed by Tetracycline (93.18%, 41/44), Ampicillin (88.64%, 39/44), Trimethoprim (47.73%, 21/44), Nalidixic acid (22.73%, 10/44), Gentamicin/Colistin (6.82%, 3/44), Tigecycline (4.55%, 2/44) and Cefotaxime (2.27%, 1/44) respectively.  The highest rate of Salmonella spp. resistance was found in Azithromycin and Sulfamethoxazole (100%, 38/38) followed by Tetracycline (60.53%, 23/38), Ciprofloxacin (47.37%, 18/38), Ampicillin (43.24%, 16/38), Nalidixic acid (34.21%, 13/38), Trimethoprim (31.58%, 12/38), Colistin (15.79%, 6/38), Gentamicin (7.89%, 3/38), Tigecycline and Cefotaxime (5.26%, 2/38) and the least is Ceftazidime (2.63%), 1/38) respectively. This information can be used to guide the use of antimicrobials in livestock farms and household farming.

ເຊື້ອ Salmonella spp. ແລະ Escherichia coli  ແມ່ນມີແນວໂນມໃນການຕ້ານຕໍ່ຢາຕ້ານສູງຂື້ນ ແລະ ຕ້ານຕໍ່ຢາຕ້ານເຊື້ອຫຼາຍສະນິດ.ວິທີມາດຕະຖານໃນການກວດຫາເຊື້ອຈຸລະຊີບທີ່ຕ້ານຕໍ່ຢາຕ້ານເຊື້ອແມ່ນມາດຕະຖານ ISO 6579-1:2017 ສໍາລັບເຊື້ອ  Salmonella spp ແລະ EFSA 2008 ສໍາລັບເຊື້ອ Escherichia coli, ມີຈຸດປະສົ່ງເພືີ່ອຕິດຕາມການນໍາໃຊ້ຢາຕ້ານເຊື້ອໃນສັດປີກ(ໄກ່)ໂດຍບໍ່ສົມເຫດສົມຜົນ, ວິທິການແມ່ນເກັບຕົວຢ່າງຈາກໄສ້ຕິ່ງໄກ່ຂອງ 144 ທີ່ມີສຸຂະພາບແຂງແຮງດີ, ທີ່ຂາຍຢູ່ໃນຕະຫຼາດ ແລະ ຈຸດຂ້າສັດ. ສະຖານທີ່ແມ່ນບ້ານ ພູເຫຼັກຈະເລີນ ແລະ ບ້ານ ພູຊ້າງຄໍາບໍ່ ນະຄອນຫຼວງພະບາງ, ແຂວງ ຫຼວງພະບາງ. ນໍາມາປູກເຊື້ອຜ່ານອາຫານລ້ຽງເຊື້ອສະເພາະໄລ່ຍະເວລາ 4 ວັນເພື່ອໃຫ້ໄດ້ເຊື້ອບໍລິສຸດ ແລະ ນໍາມາທົດສອບຢາຕ້ານຈຸລະຊີບ 14 ຊະນີດໂດຍໃຊ້ເຄື່ອງ thermo scientific: Sensititre Vizion,

 ຜົນໄດ້ຮັບໃນການສຶກສາພົບວ່າ: ໃນຕົວຢ່າງ 144 ຕົວຢ່າງກວດພົບເຊື້ອEscherichia coli  139 ຕີເປັນ 96,53%  salmonella spp 53 ຕີເປັນ 36,81%,ຜົນຂອງເຊື້ອຈຸລະຊີບທິມີແນວໂນມໃນການຕ້ານຕໍ່ຢາຕ້ານເຊື້ອມີ : ເຊື້ອ Escherichia coli ມີການຕ້ານຕໍ່ຢາ Azithromycin, Ciprofloxacin ແລະ Sulfamethoxazole ມີອັດຕາການຕ້ານສູງທີ່ສຸດເຖີງ 100% (44/44) ຮອງລົງມາໄດ້ແກ່ຢາກຸ່ມ Tetracycline (93.18%, 41/44), Ampicillin (88.64%, 39/44), Trimethoprim (47.73%, 21/44), Nalidixic acid (22.73%, 10/44), Gentamicin ແລະ Colistin (6.82%, 3/44), Tigecycline (4.55%, 2/44)ແລະ Cefotaxime (2.27%, 1/44) ຕາມລໍາດັບ. ແລະ ເຊື້ອ Salmonella spp. ມີການຕ້ານຕໍ່ຢາ Azithromycin ແລະ Sulfamethoxazole ມີອັດຕາການຕ້ານສູງທີ່ສຸດເຖີງ 100% (38/38) ຮອງລົງມາໄດ້ແກ່ຢາກຸ່ມ Tetracycline (60.53%, 23/38), Ciprofloxacin (47.37%, 18/38) Ampicillin (43.24%, 16/37), Nalidixic acid (34.21%, 13/38), Trimethoprim (31.58%, 12/38), Colistin (15.79%, 6/38), Gentamicin (7.89%, 3/38),  Tigecycline ແລະ Cefotaxime (5.26%, 2/38) ແລະ ໜ້ອຍສຸດແມ່ນ Ceftazidime (2.63%, 1/38) ຕາມລໍາດັບ, ຂໍ້ມູນນີ້ເປັນສາມາດໃຊ້ຕິດໃນການແພ່ກະຈ່າຍການນຳໃຊ້ຢ່ຕ້ານເຊື້ອຈຸລະຊີບໃນຟາມລ້ຽງສັດ ແລະ ການລ້ຽງແບບຄອບຄົວ.